By Dr. Betty Martini
Mission Possible International
9270 River Club Parkway
Duluth, Georgia 30097
Telephone: 770-242-2599
Web Site:

Posted: 25 January 2006


The effect of aspartame metabolites on human erythrocyte membrane acetylcholinesterase activity. Tsakiris S Giannoulia-Karantana A Simintzi I Schulpis KH.

Department of Experimental Physiology, Medical School, University of Athens, Greece.

Studies have implicated aspartame (ASP) with neurological problems. The aim of this study was to evaluate acetylcholinesterase (AChE) activity in human erythrocyte membranes after incubation with the sum of ASP metabolites, phenylalanine (Phe), methanol (met) and aspartic acid (aspt), or with each one separately. Erythrocyte membranes were obtained from 12 healthy individuals and were incubated with ASP hydrolysis products for 1 h at 37 degrees C. AChE was measured spectrophotometrically. Incubation of membranes with ASP metabolites corresponding with 34 mg/kg, 150 mg/kg or 200 mg/kg of ASP consumption resulted in an enzyme activity reduction by -33%, -41%, and -57%, respectively. Met concentrations 0.14 mM, 0.60 mM, and 0.80 mM decreased the enzyme activity by -20%, -32% or -40%, respectively. Aspt concentrations 2.80 mM, 7.60 mM or 10.0 mM inhibited membrane AChE activity by -20%, -35%, and -47%, respectively. Phe concentrations 0.14 mM, 0.35 mM or 0.50mM reduced the enzyme activity by -11%, -33%, and -35%, respectively. Aspt or Phe concentrations 0.82 mM or 0.07 mM, respectively, did not alter the membrane AChE activity. It is concluded that low concentrations of ASP metabolites had no effect on the membrane enzyme activity, whereas high or toxic concentrations partially or remarkably decreased the membrane AChE activity, respectively. Additionally, neurological symptoms, including learning and memory processes, may be related to the high or toxic concentrations of the sweetener metabolites.

PMID: 16129618 [PubMed - in process]


Display Summary Brief Abstract Citation MEDLINE XML UI List LinkOut ASN.1 Related Articles Cited Articles Cited in Books CancerChrom Links Domain Links 3D Domain Links GEO DataSet Links Gene Links Gene (GeneRIF) Links Genome Links Project Links GENSAT Links GEO Profile Links HomoloGene Links Nucleotide Links OMIA Links OMIM Links BioAssay Links Compound Links Compound via MeSH Substance Links Substance via MeSH PMC Links Cited in PMC PopSet Links Probe Links Protein Links SNP Links Structure Links UniGene Links UniSTS Links Show 5 10 20 50 100 200 500 Sort by Author Journal Pub Date Send to Text File Printer Clipboard E-mail Order


Note from Martini: More information on aspartame on and Aspartame Toxicity Center, I wonder how many independent studies on aspartame it takes to get the FDA to act. They have shown no concern with the 3 year study in Italy ( ) which showed aspartame causes leukemia, lymphoma, cancer of the kidney and cancer of the cranial peripheral nerves. It was peer reviewed by 7 world experts. In September at a conference the Italian researchers declared aspartame to be a multipotential carcinogen, dose related. Neurosurgeon Russell Blaylock, M.D. who wrote Excitotoxins: The Taste That Kills said mothers and those using aspartame should be terrified. Only the rats fed aspartame got malignant brain tumors. We are taking aspartame brain tumor cases from New York, New Jersey, Mississippi and Madison County, Illinois.

The problems this new study exposes are documented in Excitotoxins as well as Aspartame Disease: An Ignored Epidemic, by H. J. Roberts, M.D. We've known about them for a quarter of a century and independent studies continue to confirm. Memory loss, confusion, brain fog, blood disorders and neurological findings are well known. And the manufacturers of aspartame continue to tell whoppers:

The last thing the FDA is concerned about is safe food and drugs as they continue to turn a blind eye and deaf ear to the horrors triggered by this neuroexcitotoxic carcinogen. A study several years ago in Norway showed aspartame destroys the brain, especially in the areas of learning. The FDA is nothing more than an extension of Big Pharma, and serves to defend and protect them. It should be disbanded so an agency concerned with the health and welfare of the public can be created.

You can join the Aspartame Information List on the banner on the front page of

Dr. Betty Martini
Founder, Mission Possible International
9270 River Club Parkway
Duluth, Georgia 30097

Aspartame Toxiocity Center: